細(xì)胞名稱

人胰腺癌細(xì)胞Capan-2

貨物編碼

BFN60807521

產(chǎn)品規(guī)格

T25培養(yǎng)瓶x1

1.5ml凍存管x2

細(xì)胞數(shù)量

1x10^6

1x10^6

保存溫度

37℃

-198℃

運(yùn)輸方式

常溫保溫運(yùn)輸

干冰運(yùn)輸

安全等級(jí)

1

用途限制

僅供科研        2類

培養(yǎng)體系

90%DMEM+10%FBS+1%雙抗 

培養(yǎng)溫度

37℃

二氧化碳濃度

5%

簡(jiǎn)介

人胰腺癌細(xì)胞Capan-2取自56歲男性供體。該細(xì)胞源于ATCC

注釋

Part of: Cancer Cell Line Encyclopedia (CCLE) project.

Part of: COSMIC cell lines project.

Part of: NCI RAS program mutant KRAS cell line panel.

From: Memorial Sloan Kettering Cancer Center; New York; USA.

Registration: Memorial Sloan Kettering Cancer Center Office of Technology Development; SK2000-049.

Doubling time: 96 hours (PubMed=3019537); 45-60 hours (CLS); ~50-70 hours (DSMZ).

Microsatellite instability: Stable (MSS) (Sanger).

Omics: Array-based CGH.

Omics: Deep antibody staining analysis.

Omics: Deep exome analysis.

Omics: Deep proteome analysis.

Omics: Deep RNAseq analysis.

Omics: DNA methylation analysis.

Omics: Metabolome analysis.

Omics: Proteome analysis by 2D-DE/MS.

Omics: SNP array analysis.

Omics: Transcriptome analysis.

Caution: TP53 mutation indicated incorrectly as being at c.818G>A in PubMed=1630814.

Misspelling: CANPAN-2; In Cosmic 2434087.

基因突變

Heterozygous for KRAS p.Gly12Val (c.35G>T) (PubMed=8026879; PubMed=8426738; PubMed=11169959; PubMed=15367885).

TP53 p.Thr125Thr (c.375G>T); impairs TP53 splicing dramatically (PubMed=8026879; PubMed=24700732).

HLA信息

/

STR信息

Amelogenin        X

CSF1PO        11,12

D3S1358        17,18

D5S818        11,12

D7S820        9,11

D8S1179        12,13

D13S317        11,12 (ATCC; CLS; Cosmic-CLP; KCLB; PubMed=25877200)

11,12,13 (DSMZ)

D16S539        9,13

D18S51        13

D21S11        31

FGA        21,24

Penta D        13,15

Penta E        11

TH01        9.3

TPOX        8

vWA        17 (ATCC; CLS; Cosmic-CLP; PubMed=25877200)

16,17 (DSMZ)

17,19 (KCLB)

參考文獻(xiàn)

PubMed=27259358; DOI=10.1074/mcp.M116.058313

Humphrey E.S., Su S.-P., Nagrial A.M., Hochgrafe F., Pajic M., Lehrbach G.M., Parton R.G., Yap A.S., Horvath L.G., Chang D.K., Biankin A.V., Wu J., Daly R.J.

Resolution of novel pancreatic ductal adenocarcinoma subtypes by global phosphotyrosine profiling.

Mol. Cell. Proteomics 15:2671-2685(2016)

PubMed=27397505; DOI=10.1016/j.cell.2016.06.017

Iorio F., Knijnenburg T.A., Vis D.J., Bignell G.R., Menden M.P., Schubert M., Aben N., Goncalves E., Barthorpe S., Lightfoot H., Cokelaer T., Greninger P., van Dyk E., Chang H., de Silva H., Heyn H., Deng X., Egan R.K., Liu Q., Mironenko T., Mitropoulos X., Richardson L., Wang J., Zhang T., Moran S., Sayols S., Soleimani M., Tamborero D., Lopez-Bigas N., Ross-Macdonald P., Esteller M., Gray N.S., Haber D.A., Stratton M.R., Benes C.H., Wessels L.F.A., Saez-Rodriguez J., McDermott U., Garnett M.J.

A landscape of pharmacogenomic interactions in cancer.

Cell 166:740-754(2016)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747

Dutil J., Chen Z., Monteiro A.N., Teer J.K., Eschrich S.A.

An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.

Cancer Res. 79:1263-1273(2019)

PubMed=31037374; DOI=10.1007/s00216-019-01814-1

Lagies S., Schlimpert M., Braun L.M., Kather M., Plagge J., Erbes T., Wittel U.A., Kammerer B.

Unraveling altered RNA metabolism in pancreatic cancer cells by liquid-chromatography coupling to ion mobility mass spectrometry.

Anal. Bioanal. Chem. 411:6319-6328(2019)

PubMed=31068700; DOI=10.1038/s41586-019-1186-3

Ghandi M., Huang F.W., Jane-Valbuena J., Kryukov G.V., Lo C.C., McDonald E.R. III, Barretina J., Gelfand E.T., Bielski C.M., Li H., Hu K., Andreev-Drakhlin A.Y., Kim J., Hess J.M., Haas B.J., Aguet F., Weir B.A., Rothberg M.V., Paolella B.R., Lawrence M.S., Akbani R., Lu Y., Tiv H.L., Gokhale P.C., de Weck A., Mansour A.A., Oh C., Shih J., Hadi K., Rosen Y., Bistline J., Venkatesan K., Reddy A., Sonkin D., Liu M., Lehar J., Korn J.M., Porter D.A., Jones M.D., Golji J., Caponigro G., Taylor J.E., Dunning C.M., Creech A.L., Warren A.C., McFarland J.M., Zamanighomi M., Kauffmann A., Stransky N., Imielinski M., Maruvka Y.E., Cherniack A.D., Tsherniak A., Vazquez F., Jaffe J.D., Lane A.A., Weinstock D.M., Johannessen C.M., Morrissey M.P., Stegmeier F., Schlegel R., Hahn W.C., Getz G., Mills G.B., Boehm J.S., Golub T.R., Garraway L.A., Sellers W.R.

Next-generation characterization of the Cancer Cell Line Encyclopedia.

Nature 569:503-508(2019)